Study of genetic diversity of bovine leukemia virus isolates obtained in the Belgorod region

Abstract. The purpose of the research Was to study genetic diversity bovine leukemia virus isolates obtained in Belgorod Region using restriction fragment length polymorphism method (RFLP). Scientific novelty. Bovine leukosis is one most common chronic infectious diseases cattle many countries world, which causes significant economic losses livestock industry. typing (BLV), its structure, evaluation mutation vector and a more detailed disclosure biological properties pathogen represent fundamental applied value. Methods. object was 3-4-year-old cows infected with (n = 10), identified by serological methods disadvantaged dairy farms. immunodiffusion (ID) test, hematologic studies, PCR, genotyping, statistical processing data were conducted. Results. Conducted hematological studies determined stage leukemic process each animal. target env BLV gene (444 bp) amplificated 2-stage nested this region genotyped for all studied (RFLP) method. In course work, specific regions (gp51) gene, 970 bp long, also obtained. We have given primary assessment establishment group (Belgian genotype according RFLP). gp51 444 These fragments will be used further DNA sequencing followed phylogenetic analysis determination amino acid changes structure surface glycoprotein virus. Monitoring genotypes antigenic allow timely development latest means controlling restricting spread improvement diagnostic PCR test systems.